產(chǎn)品介紹：

Kerafast貨號：EST203 Anti-tdTomato [16D7] Antibody該抗體針對重組蛋白而產(chǎn)生，可識別 tdTomato。

tdTomato 蛋白來源于 DsRed 的單體突變體，這是一種來自 Discosoma 屬的所謂圓盤珊瑚的紅色熒光蛋白。 tdTomato 是生物技術(shù)和細(xì)胞生物學(xué)中的重要工具，可作為光譜上不同的伴侶或綠色熒光蛋白（GFP；來自 Aequorea 水母）的替代品。

Kerafast EST203 Anti-tdTomato [16D7] Antibody：This antibody was raised against a recombinant protein and recognizes tdTomato.

The tdTomato protein was derived from a monomeric mutant of DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. tdTomato is an important tool in biotechnology and cell biology as a spectrally distinct companion or substitute for the green fluorescent protein (GFP; from Aequorea jellyfish).

Product Type:	Antibody
Antigen:	Raised against a recombinant protein and recognizes tdTomato.
Isotype:	IgG2a
Clonality:	Monoclonal
Clone Name:	16D7
Reactivity:	tdTomato (also recognizes mCherry)
Species Immunized:	Rat
Purification Method:	Protein G
Buffer:	0.1M Sodium Phosphate, pH 7.4, 0.15M NaCl, 0.05% (w/v) Sodium Azide
Tested Applications:	WB (1:1000), ELISA, IF, IHC (1:100-500), IP (1ug antibody to 100ug total protein)
Storage:	4C, -20C for long term storage
Shipped:	Cold packs

數(shù)據(jù)展示：

Immunohistochemistry using Anti-tdTomato [16D7] Antibody in a transgenetic mouse, weakly expressing Cre-driven tdTomato.

The Anti-tdTomato [16D7] Antibod with Alexa-555 secondary was used to visualize Cre+ cells for tdTomato expression. The protocol used is as follows: (1) Perfuse mouse with ~ 25 ml ice-cold normal ringer solution with pH 7.4. (2) Perfuse mouse with ~ 50 ml ice-cold fixative consisting of 2% PFA and 15% picric acid with pH = 7.4. (3) After brain extraction, post fix for one hour with 2% PFA + 15% picric acid. (4) Quickly wash brain 3x with liberal amount of PBS, thoroughly removing all fixative. (5) Slice in ice-cold ringer and section at 60 µm. Wash section 3x with 0.01M PBS, 15’ each time. (6) Block for 1 hours with 10% normal goat serum INGS), 3% Bovine serum albumin (BSA), and 0.1% Trixton-X in 0.01M PBS. (7) Incubate primary tdTomato Ab at 1:500 – 1:250 for 24 hours at 4°C on a shaker. (8) Extract primary solution without washing. (9) Incubate for 2 hours in room temperature secondary (Alexa-555) at 1:500 in the following solution made in 0.01M PBS: 1% NGS + 0.01% Triton-X. (10) Wash sections 3x, 15’ each time. (11) Mount section on microscope slide and cover slip using anti-fading preservative (e.g. Vectashield or Fluoromount-G). Image courtesy of Kerafast customer Vivian Hernandez from Dr. C. Savio Chan's Lab, Northwestern University, submitted February 23, 2015.

參考文獻：

1. Shwartz Y, Gonzalez-Celeiro M, Chen CL, et al. Cell Types Promoting Goosebumps Form a Niche to Regulate Hair Follicle Stem Cells [published online ahead of print, 2020 Jul 15]. Cell. 2020;S0092-8674(20)30808-4.

2. Kim GB, Rincon Fernandez Pacheco D, Saxon D, Yang A, Sabet S, Dutra-Clarke M, Levy R, Watkins A, Park H, Abbasi Akhtar A, Linesch PW, Kobritz N, Chandra SS, Grausam K, Ayala-Sarmiento A, Molina J, Sedivakova K, Hoang K, Tsyporin J, Gareau DS, Filbin MG, Bannykh S, Santiskulvong C, Wang Y, Tang J, Suva ML, Chen B, Danielpour M, Breunig JJ. Rapid Generation of Somatic Mouse Mosaics with Locus-Specific, Stably Integrated Transgenic Elements. Cell. 2019 Sep 19;179(1):251-267.e24.

3. Aros CJ, Vijayaraj P, Pantoja CJ, et al. Distinct Spatiotemporally Dynamic Wnt-Secreting Niches Regulate Proximal Airway Regeneration and Aging [published online ahead of print, 2020 Jul 20]. Cell Stem Cell. 2020;S1934-5909(20)30286-1.

4. Locatelli G, Theodorou D, Kendirli A, Jord?o MJC, Staszewski O, Phulphagar K, Cantuti-Castelvetri L, Dagkalis A, Bessis A, Simons M, Meissner F, Prinz M, Kerschensteiner M. Mononuclear phagocytes locally specify and adapt their phenotype in a multiple sclerosis model. Nat Neurosci. 2018 Sep;21(9):1196-1208.

5. Li C, Navarrete J, Liang-Guallpa J, Lu C, Funderburk SC, Chang RB, Liberles SD, Olson DP, Krashes MJ. Defined Paraventricular Hypothalamic Populations Exhibit Differential Responses to Food Contingent on Caloric State. Cell Metab. 2018 Nov 13.

靶點科技（北京）有限公司現(xiàn)貨庫存kerafast的貨號EST203 Anti-tdTomato [16D7] Antibody。同時，這個抗體也是Anti-mCherry [16D7] Antibody，貨號是EST202.